黄郁葱，简纪常，吴灶和，鲁义善，王 蓓
（ 广东海洋大学水产学院，广东省水产经济动物病原生物学及流行病学重点实验室，
广东省水产经济动物病害控制重点实验室，湛江 524025）

摘要：从患病方斑东风螺（Babylonia areolata）分离到一株致病菌Balo001，运用生理生化方法并结合分子生物学方法对病原菌进行分类鉴定，并对病原菌进行药敏分析及LD50测定。结果表明，致病菌Balo001为哈氏弧菌（Vibrio harveyi），它对东风螺的LD50值为6.8×105 CFU/g；药敏试验结果表明该菌对氟哌酸、氟苯尼考、复方新诺明等抗菌药有较高的敏感性。

关键词：方斑东风螺；吻管水肿病；哈氏弧菌

Preliminary study on the pathogenic bacteria isolated from
proboscis edema-diseased Babylonia areolata
Huang Yu-cong, Jian Ji-chang, Wu Zao-he, Lu Yi-shan, Wang Bei
( Fisheries College of Guangdong Ocean University,
Guangdong Province Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Key Laboratory of Diseases Control for Aquatic Economic Animals, Zhanjiang 524025, China)

Abstract: An epidemic proboscis edema disease of Babylonia areolata occurred in Zhanjiang in October of 2005. A pathogen named Balo001 isolated from moribund Babylonia areolata by aseptic methods, we have made use of physiological and biochemical methods combined with molecular biology methods to classify and identify the pathogen, and drug sensitivity analysis of pathogens as well as LD50 value were tested too. The results showed that the pathogen named Balo001 was Vibrio harveyi. The LD50 of the pathogen to Babylonia areolata was 6.8×105 CFU/g. Antibiotic sensitivity analysis indicated that the pathogen was sensitive to norfloxacin, florfenicol, compound Sulfamcthoxazolc. This showed that the pathogenic bacteria has strong toxiferous to Babylonia areolata, it can lead to the proboscis edema disease of Babylonia areolata. The results of the research provide a theoretical basis for prevention and control of the proboscis edema of Babylonia areolat.

Key words: Babylonia areolata; proboscis edema; Vibrio harveyi

基金项目：广东省科技计划项目(2005B60301014；2008A060201012)；湛江市科技项目
作者简介：黄郁葱（1978—），男，研究实习员，硕士，从事水产动物病害防治研究。E-mail: hyczjou@163.com
通讯作者：简纪常（1964—），男，教授，博士生导师，从事水产动物免疫学及病害控制。E-mail: jianjc@gmail.com
 

(来源：《渔业现代化》2009.36(4)：37-41)