孟  振¹，刘新富¹，雷霁霖¹，张和森²
（1 农业部海洋渔业资源可持续利用重点开放实验室，中国水产科学研究院黄海水产研究所，青岛 266071；
2 青岛通用水产养殖有限公司，青岛 266404）

摘要：提出一种快速、准确测定鲆鲽类体表面积的新方法，利用数码相机拍照获取带有标准坐标纸背景的鲆鲽类鱼体数码图像，采用图像处理软件Photoshop CS2和Adobe Acrobat Professional分别获得单位背景面积和鱼体图像的像素数，通过两者的比例计算出体表面积。30尾大菱鲆（Scophthalmus maximus）体表面积的实测表明，与传统计数方格面积法的测量结果相比，Photoshop法相对误差为－4.40%~3.08%，Acrobat法相对误差为－1.83%~1.30%。统计分析显示，2种图像处理软件所测数据与传统方法测量值间差异都不显著，测量1尾大菱鲆体表面积，传统方法约耗时15 min，Photoshop和Acrobat法分别只需3 min和1.5 min。新方法可以迅速和准确地测定鲆鲽类体表面积，便于鲆鲽类养殖生产中对养殖密度进行有效管理。

关键词：鲆鲽类；大菱鲆；体表面积；图像处理软件；数码技术　　

A new method for measuring surface area of flatfishes by digital technology
MENG Zhen¹, LIU Xin-fu¹, LEI Ji-lin¹, ZHANG He-sen²
(1 Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Ministry of Agriculture;
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China;
2 Qingdao General Aquaculture Co., Ltd, Qingdao 266404, China )

Abstract: A new method for measuring surface area of flatfishes was presented in this paper. Digital camera is used to capture digital images of flatfishes with a background of standard plotting paper, image processing softwares (Photoshop CS2 or Adobe Acrobat Professional) are used to get pixels per square centimeter and the pixels of flatfishes, then surface area of flatfishes can be calculated by these data. Compared with traditional method, results of surface area measuring of 30 turbot (Scophthalmus maximus L) revealed that relative errors of Photoshop Method and Acrobat Method were －4.40% to 3.08% and －1.83% to 1.30%, respectively. Statistical analysis revealed there were no significant differences between the two software measuring methods and the traditional measuring method, respectively. It takes about 15 min for measuring one turbot, while Photoshop Method and Acrobat Method only need 3 min and 1.5 min, respectively. These results indicated that the new method could provide a convenient and accurate measuring method for surface area of flatfishes, which is very useful to calculate surface coverage by the culture flatfishes.

Key words: Flatfishes; turbot; Scophthalmus maximus L.; surface area; Image processing
software; digital technology

基金项目：农业部公益性行业重大科研专项（nyhyzx07-046）

作者简介：孟振（1981-），男，硕士，研究实习员，主要从事海水鱼类遗传育种研究。E-mail: mengzhen@ysfri.ac.cn

通讯作者：雷霁霖（1935-），中国工程院院士，主要从事海水鱼类增养殖研究。E-mail: leijl@ysfri.ac.cn

                                       （《渔业现代化》 2008，35（4），12-15）