季 祥^1，2，王金荣¹，王新平¹，朱文祥，蔡 禄^1，2
（1 内蒙古科技大学 生物工程与技术研究所，内蒙古 包头 014010；
2 内蒙古自治区生物质能源化利用重点实验室，内蒙古 包头 014010）

摘要：为了提高可作为生物质能源微藻原料的富油斜生栅藻（Scenedesmus obliquus）的生物量，以BG11培养基为基础培养基，对C、N、P等 3种营养盐进行了优化，采用了单因素和L9(33)正交试验进行优化。结果表明：异养培养斜生栅藻的最适碳源为葡萄糖；最适氮源为硝酸钠；3种营养盐最佳水平组合为：葡萄糖浓度为20 g/L、NaNO3浓度为2.0 g/L、K2HPO4•3H2O浓度为0.15 g/L，富油斜生栅藻在优化后的培养基中生长情况良好，稳定期最大生物量（A680）））可达10.276，可作为后续生物柴油生产的原料。
关键词：斜生栅藻；微藻生物柴油；异养培养；培养条件优化

Heterotrophic culture of high-oil Scenedesmus obliquus and
optimization of growth conditions
JI Xiang, WANG Jin-rong, WANG Xin-ping, ZHU Wen-xiang, CAI Lu
( 1 Institute of Bioengineering and Technology, Inner Mongolia University of Science and Technology, Baotou Inner Mongolia 014010, China; 2 Inner Mongolia Key Laboratory of Biomass-Energy Conversion, Baotou Inner Mongolia 014010, China )

Abstract: In this paper, BG11 medium was chosen as basal medium. The cultivating concentrations of three kinds of nutrients: C, N, P were optimized, using a single factor experiment and L9 (33) orthogonal design method. The results showed, in the heterotrophic culture of Scenedesmus obliquus, the glucose was the best carbon source, the sodium nitrate was best nitrogen source and the optimal concentrations of three kinds of nutrients was: glucose 20 g/L, NaNO3 2.0 g/L, K2HPO4•3H2O. High-oil Scenedesmus obliquus grew very well in optimized medium and the maximum biomass (A680) in the stationary phase was up to 10.276. These showed the high-oil Scenedesmus obliquus may be a raw material of the biodiesel production.
Key words: Scenedesmus obliquus; microalgae biodiesel; heterotrophic culture; growth condition optimization

基金项目：教育部春晖计划（Z2009-1-01057）；内蒙古自然科学基金（2010MS0521）；内蒙古科技大学创新基金项目（2009NC061）。
作者简介：季祥（1978－），男，副教授，从事生物质能、生物制浆研究。E-mail: nmjixiang@sina.com
通讯作者：蔡禄（1964－），男，教授，主要从事生物质能、生物信息、分子生物学研究。E-mail: nmcailu@163.com。
 

（《渔业现代化》2011.38（5）：1-6）