王茂林，李 岑，杨 敏，杨文钰，姜玉声，包鹏云
（大连海洋大学，农业部海洋水产增养殖学重点开放实验室，辽宁大连 116023）
摘要：在水温12~15 ℃下采用实验生态法研究了盐度（10、15、20、25、30、35、40和42）对体质量3.0 g的仿刺参（Apostichopus japonicus）存活参肠、呼吸树Na+ -K+-ATP酶活性的影响。48 h盐度急性实验表明：仿刺参存活适宜盐度范围为。相同盐度时不同时间仿刺参Na+ -K+-ATP酶活性测定试验表明：盐度为20、33时肠和呼吸树的Na+ -K+-ATP酶活性先急剧下降后回升，最后稳定；盐度为28（暂养的海水）时，肠和呼吸树的Na+ -K+-ATP酶一直保持稳定值。时间相同时不同盐度下仿刺参Na+ -K+-ATP酶活性测定试验表明：6 h时，盐度越接近28，肠和呼吸树的Na+ -K+-ATP酶活性越高，盐度低于20或高于35，肠和呼吸树的Na+ -K+-ATP酶活性急速降低；24 h时，在盐度20-35范围内，盐度越高，肠和呼吸树的Na+ -K+-ATP酶活性越高，而此范围之外，肠和呼吸树的Na+ -K+-ATP酶活性严重下降。
关键词：刺参；盐度；肠；呼吸树；渗透调节；Na+ -K+-ATP酶
Effects of salinity on osmotic adjustment of sea cucumber
WANG Maolin, LI Cen, YANG Min, YANG Wenyu, JIANG Yusheng, BAO Pengyun
( Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Ocean University, Dalian 116023 , China )
Abstract: Salinity influences on the survival of the sea cucumber(Apostichopus japonicus) of 3.0g and the Na+/K+-ATPase activity in intestines and respiratory trees were carried out under 12-15℃, and were divided into 8 different salinity groups(10, 15, 20, 25, 30, 35, 40 and 42). Results of acute salinity test after 48h showed that the suitable survival salinity was 20-35. Na+/K+-ATPase activity tests under the same salinity for different time showed that, the Na+/K+-ATPase activity decreased rapidly and tended stable after a short increase under the salinity of 20 and 33, while it remained stable under 28. Results of Na+/K+-ATPase activity tests under different salinity for the same time were as follows: for 6h, Na+/K+-ATPase activity increased as the salinity was close to 28, while decreased rapidly below 25 or above 35; for 24h, Na+/K+-ATPase activity increased as the salinity increased under 20-35, while decreased rapidly outside this range.
Key words: sea cucumber; salinity; intestine; respiraory tree; osmoregulation; Na+-K+-ATPase enzyme
（来源：《渔业现代化》2014,41(1):6-9）
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