毛国民¹，楼 宝¹，骆季安¹，史会来¹ ，辛 俭¹，郑代明¹，史海东²
（1 浙江省海洋水产研究所，舟山 316100；2 浙江普陀区海洋与渔业局，舟山 316100）

摘要：试验研究了舟山牙鲆人工培育及促熟催产中的技术措施。采用自然海区捕获的牙鲆亲鱼，逐年筛选、精心培育，共获得健康的2 ~ 4 龄成熟亲鱼300尾（雄鱼200尾、雌鱼100尾），体重1 000~5 000 g。在自然产卵和外源激素HCG（1 500 IU/kg）及LHRH-A3（5 μg/kg）的作用下（雄鱼减半），共采卵536.2×104 粒，上浮率51.3%，受精率69.5%，孵化率84.2%。与自然产卵相比，激素催产产卵数量多，受精率和孵化率相对偏低；而自然产卵受精率和孵化率高，但产卵不够集中且数量相对较少。

关键词：舟山褐牙鲆；亲鱼培育；催产；人工繁殖
 

Study on broodstock cultivation and artificial propagation technique of ZhouShan Flounder (Paralichthys olivaceus)
MAO Guo-min, LOU Bao, LUO Ji-an, SHI Hui-lai, XIN Jian, ZHENG Dai-ming, SHI Hai-dong
( 1. ZheJiang Marine Fisheries Research Institute, Zhoushan 316100, China; 2 .Pu Tuo Oceanic and Fishery Administration, Zhoushan 316100, China )

Abstract: In this paper, broodstock cultivation and artificial propagation technique of paralichthys olivaceus were studied. Three hundred 2~4 years matured broodstock ( female 200, male 100 ) were obtained from natural sea area by effective selecting gradually, which the body weight were varied between 1 000g and 5 000g. By the method of spawning naturally and injecting the hormone HCG ( 1500 IU/kg ) and LHRH-A3 (5μg/kg ) in female broodstock, but HCG and LHRH-A3 halved in male broodstock, 536.2×104 eggs were gained. The float-rate, fertilization-rate, and hatch-rate were 51.3%, 69.5%, and 84.2% respectively. Compared with natural spawning, there were more eggs by injecting the hormone, while the fertilization-rate and hatch-rate were lower. Though the fertilization-rate and hatch-rate were high, natural spawning had fewer eggs and finished time after time.

Key words: Zhoushan paralichthys olivaceus; broodstock cultivation; propagation; artificial reproduction

基金项目：浙江省科技计划项目（2006C22038）
作者简介：毛国民（1958—），工程师，主要从事海水鱼类增养殖技术研究。E-mail:shihuilai1980@163.com
 

（来源：《渔业现代化》 2008，35（1），36-38）