胡惠平¹，刘 源¹，孙晓红¹，潘迎捷¹，孙 湛²，朱丽敏²，赵 勇¹*
（1 上海海洋大学食品学院，上海 201306；2 Alpha M.O.S., Toulouse France 31400，亚太办事处，上海 200336）

摘要：为了将电子鼻技术应用于水产品中副溶血性弧菌等食源性致病菌的快速无损检测，试验利用电子鼻对从南美白对虾（Litopenaeus vannamei）中分离的一株副溶血性弧菌（Vibrio parahaemolyticus）进行检测。试验采用具有18个金属氧化物传感器的电子鼻对副溶血性弧菌经纯培养所得的挥发性代谢产物进行检测，同时用空白3.5%氯化钠TSB培养液作为对照。电子鼻所得的数据用主成分分析（PCA）、判别因子分析（DFA）、单类成分判别分析（SMICA）等多元统计方法进行数据分析。结果显示，副溶血性弧菌经纯培养后产生了明显不同于空白培养液气味的挥发性代谢产物，表明电子鼻可以应用于纯培养微生物的检测。

关键词：电子鼻；副溶血性弧菌；挥发性代谢产物

Using Electronic Nose to Detection one strain of Vibrio parahaemolyticus isolated from Litopenaeus vannamei
HU Hui-ping¹, LIU Yuan¹, SUN Xiao-hong¹,
SUN Zhan², ZHU Li-min², PAN Ying-jie¹, ZHAO Yong¹*
( 1 College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2 Alpha M.O.S., Toulouse, France 31400, Asia Pacific Representative Office, Shanghai 200336, China )

Abstract: Use of electronic nose to rapid and non-destructive detection one strain of Vibrio parahaemolyticus from Litopenaeus vannamei. An electronic nose with 18 metal oxide sensors was used to monitor the volatile profiles produced by Vibrio parahaemolyticus. 3.5% sodium chloride (TSB) without inoculation of Vibrio parahaemolyticu was used as control. Principal component analysis (PCA), discriminant function analysis (DFA) and soft independent modeling of class analogy (SMICA) were used as pattern recognition tools. The result showed that the volatile profiles from Vibrio parahaemolyticus were distinctly different from that of tryptic soy broth. It suggests that there have the potential for application of electronic nose in detection of microorganism-inoculated.

Key words: Vibrio parahaemolyticus; volatile profiles; electronic nose

基金项目：上海市青年科技启明星计划资助（07QA14047）；上海市科技兴农重点攻关项目（沪农科攻字2005第4-2号）作者简介：胡惠平（1982—），女，在读硕士研究生，研究方向：食品安全。E-mail: huhuiping0223112@126.com
通讯作者：赵勇（1975—），男，副教授，硕士研究生导师，研究方向：食品生物技术、食品安全。E-mail: yzhao@shou.edu.cn
沪农科攻字2006第10-5号）；上海市创新行动计划（08390513900）
 

(来源：《渔业现代化》2009.36(3)：41-44,48)