黄翔鹄 ^1，2，刘 梅²，周美华¹，古滨河³
（1 东华大学环境科学与工程学院，上海 201620；
2 广东海洋大学水产学院，广东 湛江 524025；
3 美国佛罗里达大学土壤和水科学系，美国 佛罗里达州 32611）

摘要：利用15N稳定同位素标记物，研究在不同盐度下波吉卵囊藻（Oocystis borgei）对溶解态氮的吸收速率和选择性。结果表明：盐度对波吉卵囊藻氮吸收速率影响显著（P<0.05）。当盐度为15时，波吉卵囊藻对氨氮（NH4＋-N）、亚硝酸盐氮（NO2－-N）、硝酸盐氮（NO3－-N）等均有较大的吸收速率，分别为1.69、0.112、0.028 μg N/(g•h)；盐度为30时，对尿素氮（Urea-N）有较大的吸收速率，为0.074 μg N/(g•h)。不同盐度下，波吉卵囊藻对4种溶解性氮的选择性吸收的先后顺序为：氨氮＞亚硝酸盐氮＞尿素氮＞硝酸盐氮。因此，可通过在对虾养殖环境中接种波吉卵囊藻，以吸收水体中过高浓度的氨氮和亚硝酸盐氮，改善虾池养殖水质，促进健康生态养殖。

关键词：波吉卵囊藻；对虾；溶解性氮；吸收速率

The uptake rate and selectivity of dissolved nitrogen by Oocystis borgei in prawn pond
HUANG Xiang-hu ^{1, 2}, LIU Mei², ZHOU Mei-hua¹, GU Bin-he ³
( 1 School of Environmental Science and Engineering, Donghua University, Shanghai 201620, China;
2 Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China;
3 Department of Soil and Water, University of Florida, Gainesville, Florida 32611, USA )

Abstract：The uptake rate of nitrogen source by Oocystis borgei was studied in this paper through adding four dissolved nitrogens and 15N in algal culture solution. The result indicated that effect of salinity on uptake rate (ρ) of nitrogen by algae was significant. When salinity was 15, the values of , and up to the maximum, were 1.691 μg N/(g•h), 0.028 μg N/(g•h), 0.112 μg N/(g•h) respectively, while reached maximum 0.074 μg N/(g•h) when salinity was 30. Meanwhile, the order of selective uptake of dissolved nitrogen was ammonium, nitrite, urea and nitrate. Therefore, we can improve the water quality of prawn pond by anding Oocystis borgei to remove ammonium and nitrite nitrogen.

Key words: Oocystis borgei; prawn; dissolved nitrogen; uptake rate

基金项目：科技部农业科技成果转化资金项目（2010GB2E000351）；广东省科技计划项目（2010B060200011，2009B020312013，2006A20305003）
作者简介：黄翔鹄（1962—），男，教授，从事水域生态研究。E-mail: hxh166@126.com

（来源：《渔业现代化》2012,39(3):34-39）