李  怡¹，曹海鹏¹，陈水祥²，杨先乐¹
（1 农业部渔业动植物病原库，上海水产大学 上海 200090；2 杭州市萧山水祥黑鱼养殖场，浙江萧山 311228）

摘要：为了探讨噬菌蛭弧菌制剂净化水质和病害防治的效果，试验以0.75 mL/m2的用量向乌鳢养殖池泼洒浓度为1.0×108 pfu/mL的噬菌蛭弧菌制剂，观察7 d内养殖池的pH、DO、NH3-N、NO2－-N含量及弧菌总数等主要水质指标以及乌鳢死亡数目的变化。向乌鳢养殖池加入噬菌蛭弧菌后，与对照组相比，试验组NH3-N、NO2－-N含量及弧菌总数明显减少（p＜0.05），DO明显增大（p＜0.05），但pH变化不明显（p＞0.05）；此外，乌鳢死亡率也明显减少（p＜0.05）。试验结果表明，以0.75 mL/m2的用量向乌鳢养殖池泼洒浓度1.0×108 pfu/mL的噬菌蛭弧菌制剂，有利于提高DO含量及乌鳢存活率，降低NH3-N、NO2－-N含量及弧菌总数。

关键词： 噬菌蛭弧菌；乌鳢；养殖水质

Effect of Bdellovibrio bacteriovorus on the water quality
of snakehead fish farming pond
LI Yi¹, CAO Hai-peng¹, CHEN Shui-xiang², YANG Xian-le¹
( 1 Aquatic Pathogen Collection Center of Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China; 2 Shuixiang farm of Ophiocephalus argus, Xiaoshan Zhejiang 311228, China )

Abstract: The experiment aimed to study the effect of Bdellovibrio bacteriovorus on water quality of farming pond and fish disease control, a concentration of 1.0×108 pfu/mL Bdellovibrio bacteriovorus was poured into the snakehead (Ophiocephalus argus) farming pond with the usage of 0.75 mL/m2, and the changes (△) of the water quality agent such as DO content, NH3-N content, NO2－-N content, Vibrio population and the death numbers of snakehead were studied for 7 days. It was demonstrated that, in contrast with the control group, △(NH3-N), △(NO2－-N ) and △(Vibrio population) in the experiment group were dramatically lowered (p＜0.05), and △(DO) was dramatically increased (p＜0.05), but the change of pH was not obvious (p>0.05). Furthermore, the average △(death numbers) of snakehead was also reduced (p＜0.05). The experiment results indicated that the use of 1.0×108 pfu/mL Bdellovibrio bacteriovorus with the usage of 0.75 mL/m2 helped to increase the content of DO of farming pond and survival rate of snakeheads, and also helped to accelerate the reduction of the content of NH3-N, NO2－-N and Vibrio population in the farming pond.

Key words: Bdellovibrio bacteriovorus; snakehead fish; ophiocephalus argus; ater quality

基金项目：上海市优秀青年教师科研专项基金（B-8101-08-0017）, 上海水产大学青年科研基金（A-0212-07-0251）

作者简介：李怡（1978－），女，助理实验师，主要从事水产动物病害研究。E-mail: hpcao@shfu.edu.cn
                                      
                                         

 （来源：《渔业现代化》 2008，35（2））