石芳永¹，张延青¹，徐  洋¹，刘  鹰 ²，刘明星²
（1 青岛理工大学，青岛　266033； 2中国科学院海洋研究所，青岛 266071）

摘  要：采用酒石酸钾钠溶液-氢氧化钠溶液作为屏蔽剂排除海水中钙、镁离子对测定的干扰，将纳氏试剂比色法用于对海水养殖废水中氨氮的测定，最佳测定条件为200 g/L的氢氧化钠溶液用量2.5 mL，纳氏试剂1.5 mL，显色时间25 min。该方法对盐度为10~32的海水可以直接测定，氨氮质量浓度在0.01~0.04 mg/L符合比尔定律，检出限为0.01 mg/L，加标回收率在91%~108%。该法操作方便、快捷，灵敏度好、显色速度快且方法稳定。

关键词：海水养殖；氨氮测定；次溴酸盐氧化法；纳氏试剂比色法

Study on the influencing factors and improvement of NH3-N
determination in Mariculture water
SHI Fang-yong¹, ZHANG Yan-qing¹, XU Yang¹, LIU Ying ², LIU Ming-xing²
( 1 Qingdao Technological University, Qingdao 2660003, China;
2 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China )

Abstract：Hypobromite Oxidation prosess can not apply to NH3-N determination in mariculture water, this is mainly because the high NH3-N concentration is out of the range of standard curve. Furthermore, the concentration of organic matters and reducing substances are too high, NH3-N can not achieve complete oxidation due to the lack of oxidant. The tests indicated that, with potassium sodium tartrate and sodium hydroxide solution as shielding agent for eliminating the interference of calcium and magnesium ions, Hypobromite Oxidation prosess can be used to determine NH3-N. The best conditions in the lab were as follows: the dosage of sodium hydroxide solution was 2.5 mL at its concentration of 200 g/L, the dosage of Nessler's Reagent was 1.5 mL, and the best color-developing time was 25 minutes. This method is suitable for sea water with salinity at 10~32. Bear's law is obeyed for NH3-N in the range of 0.01~0.04 mg/L, the detection limit is 0.01 mg/L and the recovery of standard addition is in the range of 91%~108%. This method is convenient, good sensitivity, color fast and stable, and it fully meets the requirements of NH3-N determination in Mariculture water.

Key words: mariculture water, NH3-N determination, Hypobromite Oxidation prosess, nessler's reagent colorimetry

基金项目：国家“863”高技术研发计划（2006AA100305）；国家自然科学基金项目（30671620）； 国家科技支撑计划项目（2006BAD09A03）

作者简介：石芳永（1983—），男，硕士研究生，主要从事环境工程研究。E-mail：shifangyong0806@yahoo.com.cn

通讯作者：张延青（1964—），女，教授，博士，从事水污染控制工程研究和教学。E-mail: zyq_luck@163.com。

                                               （《渔业现代化》 2009，36（2），20-24）